SEROTYPE DISTRIBUTION AND FREQUENCY OF ERM(B) AND MEF(A) GENES IN MACROLIDE-RESISTANT STREPTOCOCCUS PNEUMONIAE STRAINS COLLECTED FROM CHILDREN UNDER 5 YEARS OLD WITH PNEUMONIA IN NGHE AN
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Abstract
Objective: This study aims to determine the serotypes and frequency of carrying erm(B) and mef(A) genes in macrolide antibiotic-resistant pneumococcal strains collected from children under 5 years old with pneumonia in Nghe An.
Methods: It was a descriptive cross-sectional study on 126 pneumococcal strains from November 2019 to December 2021.
Results: Eight serotypes 6A/B, 9V, 11A, 14, 15A, 19F, 19A, and 23F were identifed in Nghe An, of which serotype 19A accounts for a high proportion. The presence of erm(B) and mef(A) genes was determined by PCR reactions, the frequency of carrying erm(B) and mef(A) genes was 92.1% and 57.9%, respectively. The frequency of carrying at least one of these two genes is 95.3% and the frequency of carrying two genes simultaneously is 54.8%. There are 6 macrolide antibiotic-resistant strains that do not carry any of the erm(B) and mef(A) genes, accounting for 4.8%.
Conclusion: Eight different serotypes of pneumococcus were identifed in Nghe An. 19F, 23F and 19A are the most common. Serotype 19A with its high infection rate is a feature that needs attention. The frequency of carrying the erm(B) and mef(A) genes is 92.1 and 57.9%, respectively.
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Keywords
serotype, erm(B), drug resistance, macrolide, mef(A), Streptococcus pneumoniae
References
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